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验证数据展示

  • WB analysis using 31301-1-AP

    WB analysis using 31301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31301-1-AP (EPS15 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 31301-1-AP

    WB analysis of MCF-7 using 31301-1-AP

    WB result of EPS15 antibody (31301-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS15 transfected MCF-7 cells.

  • Featured Product
  • KD/KO验证

EPS15 Polyclonal antibody

EPS15 Polyclonal Antibody for WB, ELISA
Cat No. 31301-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

AF 1P, AF1P, MLLT5, Protein AF 1p, Protein Eps15

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EPS15 Ready-To-Use IHC Kit. EPS15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31301-1-AP targets EPS15 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35282

Product name: Recombinant human EPS15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 700-860 aa of NM_001981.3

Sequence: VVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epidermal growth factor receptor pathway substrate 15
别名 AF 1P, AF1P, MLLT5, Protein AF 1p, Protein Eps15
计算分子量99 kDa
观测分子量140 kDa
GenBank蛋白编号NM_001981.3
基因名称 EPS15
Gene ID (NCBI) 2060
RRIDAB_3669938
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP42566
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPS15 was originally identified as a substrate for the kinase activity of the epidermal growth factor receptor (EGFR). EPS15 has a tripartite structure comprising an NH2-terminal portion, which contains three EH domains, a central putative coiled-coil region, and a COOH-terminal domain containing multiple copies of the amino acid triplet Aspartate-Proline-Phenylalanine (PMID: 10481267). EPS15 is phosphorylated on tyrosine residues following EGFR activation (PMID: 7689153).

实验方案

Product Specific Protocols
WB protocol for EPS15 antibody 31301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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