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验证数据展示

  • WB analysis of human skeletal muscle using 16421-1-AP (same clone as 16421-1-PBS)

    WB analysis of human skeletal muscle using 16421-1-AP (same clone as 16421-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 16421-1-AP (same clone as 16421-1-PBS)

    IF Staining of HeLa using 16421-1-AP (same clone as 16421-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO3 antibody (16421-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

ENO3 Polyclonal antibody, PBS Only

ENO3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16421-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Muscle-specific enolase, MSE, GSD13, EC:4.2.1.11, Beta-enolase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.

产品信息

16421-1-PBS targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9613

Product name: Recombinant human ENO3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 94-434 aa of BC017249

Sequence: MIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enolase 3 (beta, muscle)
别名 Muscle-specific enolase, MSE, GSD13, EC:4.2.1.11, Beta-enolase
计算分子量 434 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC017249
基因名称 ENO3
Gene ID (NCBI) 2027
RRIDAB_2099464
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13929
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13).

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