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验证数据展示

  • WB analysis of human skeletal muscle using 16421-1-AP

    WB analysis of human skeletal muscle using 16421-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16421-1-AP

    IF Staining of HeLa using 16421-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO3 antibody (16421-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ENO3 Polyclonal antibody

ENO3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16421-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IF/ICC, ELISA

别名

Muscle-specific enolase, MSE, GSD13, EC:4.2.1.11, Beta-enolase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, human brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

16421-1-AP targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 ENO3 fusion protein Ag9613 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enolase 3 (beta, muscle)
别名 Muscle-specific enolase, MSE, GSD13, EC:4.2.1.11, Beta-enolase
计算分子量 434 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC017249
基因名称 ENO3
Gene ID (NCBI) 2027
RRIDAB_2099464
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13929
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13).

实验方案

Product Specific Protocols
WB protocol for ENO3 antibody 16421-1-APDownload protocol
IF protocol for ENO3 antibody 16421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Cell Res

Phospholipase A2-activating protein induces mitophagy trough anti-apoptotic MCL1-mediated NLRX1 oligomerization

Authors - Zhilong Zheng
View Article
mouseWB

Mol Cell Proteomics

Extended Multiplexing of Tandem Mass Tags (TMT) Labeling Reveals Age and High Fat Diet Specific Proteome Changes in Mouse Epididymal Adipose Tissue.

Authors - Deanna L Plubell
View Article
ratWB

Cell Physiol Biochem

Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats.

Authors - Choi Minji M
View Article