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验证数据展示

  • WB analysis using 27642-1-AP (same clone as 27642-1-PBS)

    WB analysis using 27642-1-AP (same clone as 27642-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 27642-1-AP (ELA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 27642-1-AP (same clone as 27642-1-PBS)

    IHC staining of human lymphoma using 27642-1-AP (same clone as 27642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ELANE/ELA2 antibody included
  • IHC staining of human lymphoma using 27642-1-AP (same clone as 27642-1-PBS)

    IHC staining of human lymphoma using 27642-1-AP (same clone as 27642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27642-1-AP (ELA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ELANE/ELA2 antibody included
  • IF Staining of HEK-293 using 27642-1-AP (same clone as 27642-1-PBS)

    IF Staining of HEK-293 using 27642-1-AP (same clone as 27642-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ELA2 antibody (27642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27642-1-PBS in a different storage buffer formulation.

Neutrophil Elastase Polyclonal antibody, PBS Only

Neutrophil Elastase Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27642-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ELANE, ELA2, HNE, HLE, Elastase 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ELANE/ELA2 Ready-To-Use IHC Kit. ELANE/ELA2 primary antibody included.

产品信息

27642-1-PBS targets Neutrophil Elastase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Neutrophil Elastase fusion protein Ag26634 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elastase 2, neutrophil
别名 ELANE, ELA2, HNE, HLE, Elastase 2
计算分子量 267 aa, 29 kDa
观测分子量29 kDa
GenBank蛋白编号BC074816
基因名称 ELA2
Gene ID (NCBI) 1991
RRIDAB_2918126
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08246
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.