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验证数据展示

  • WB analysis using 20456-1-AP (same clone as 20456-1-PBS)

    WB analysis using 20456-1-AP (same clone as 20456-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20456-1-AP (EI24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20456-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 20456-1-AP (same clone as 20456-1-PBS)

    IHC staining of human colon cancer using 20456-1-AP (same clone as 20456-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20456-1-AP (EI24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20456-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EI24 Polyclonal antibody, PBS Only

EI24 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20456-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

20456-1-PBS targets EI24 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14169

Product name: Recombinant human EI24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 267-340 aa of BC002390

Sequence: SILFPLFIISANEAKTPGKAYLFQLRLFSLVVFLSNRLFHKTVYLQSALSSSTSAEKFPSPHPSPAKLKATAGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 etoposide induced 2.4 mRNA
别名
计算分子量 340 aa, 39 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC002390
基因名称 EI24
Gene ID (NCBI) 9538
RRIDAB_10858326
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14681
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ei24 is an ER-localized protein originally identified as a p53-induced proapoptotic gene in etoposide-treated NIH3T3 cells. It has been shown to be able to bind to the antiapoptotic protein Bcl-2 (PMID: 15781622), play a role in autophagy (PMID: 23074225), and induce proliferate arrest/apoptosis (PMID: 10594026). Ei24 can bind specifically to IMPβ1 and IMPα2 to impede their normal role in nuclear import (PMID: 24821838).

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