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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 27875-1-AP (same clone as 27875-1-PBS)

    WB analysis using 27875-1-AP (same clone as 27875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EGLN3 antibody included
  • IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EGLN3 antibody included

EGLN3 Polyclonal antibody, PBS Only

EGLN3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27875-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

EGLN3/PHD3, PHD3, EC:1.14.11.-, EC:1.14.11.29, Egl nine homolog 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EGLN3 Ready-To-Use IHC Kit. EGLN3 primary antibody included.

产品信息

27875-1-PBS targets EGLN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27464

Product name: Recombinant human EGLN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-115 aa of BC010992

Sequence: MPLGHIMRLDLEKIALEYIVPCLHEVGFCYLDNFLGEVVGDCVLERVKQLHCTGALRDGQLAGPRAGVSKRHLRGDQITWIGGNEEGCEAISFLLSLIDRLVLYCGSRLGKYYVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 egl nine homolog 3 (C. elegans)
别名 EGLN3/PHD3, PHD3, EC:1.14.11.-, EC:1.14.11.29, Egl nine homolog 3
计算分子量 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC010992
基因名称 EGLN3
Gene ID (NCBI) 112399
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6Z9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

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