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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 27875-1-AP (same clone as 27875-1-PBS)

    WB analysis using 27875-1-AP (same clone as 27875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EGLN3 antibody included
  • IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    IHC staining of mouse colon using 27875-1-AP (same clone as 27875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27875-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EGLN3 antibody included

EGLN3 Polyclonal antibody, PBS Only

EGLN3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27875-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

EGLN3/PHD3, PHD3, EC:1.14.11.-, EC:1.14.11.29, Egl nine homolog 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EGLN3 Ready-To-Use IHC Kit. EGLN3 primary antibody included.

产品信息

27875-1-PBS targets EGLN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 EGLN3 fusion protein Ag27464 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 egl nine homolog 3 (C. elegans)
别名 EGLN3/PHD3, PHD3, EC:1.14.11.-, EC:1.14.11.29, Egl nine homolog 3
计算分子量 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC010992
基因名称 EGLN3
Gene ID (NCBI) 112399
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6Z9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.