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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 20894-1-AP (same clone as 20894-1-PBS)

    WB analysis using 20894-1-AP (same clone as 20894-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20894-1-AP (E-selectin/CD62E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

    IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

    IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

E-selectin/CD62E Polyclonal antibody, PBS Only

E-selectin/CD62E Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20894-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

CD62E, E-selectin, CD62 antigen-like family member E, ELAM1, ELAM 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

20894-1-PBS targets E-selectin/CD62E in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14944

Product name: Recombinant human SELE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-371 aa of BC142711

Sequence: WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFDCEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSPAGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTALS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 selectin E
别名 CD62E, E-selectin, CD62 antigen-like family member E, ELAM1, ELAM 1
计算分子量 610 aa, 67 kDa
观测分子量115 kDa
GenBank蛋白编号BC142711
基因名称 CD62E
Gene ID (NCBI) 6401
RRIDAB_10755287
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16581
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

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