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验证数据展示

  • WB analysis using 27958-1-AP (same clone as 27958-1-PBS)

    WB analysis using 27958-1-AP (same clone as 27958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27958-1-AP (DROSHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

    IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 27958-1-AP (same clone as 27958-1-PBS)

    WB analysis of HEK-293 using 27958-1-AP (same clone as 27958-1-PBS)

    WB result of DROSHA antibody (27958-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DROSHA transfected HEK-293 cells. This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

    IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DROSHA Polyclonal antibody, PBS Only

DROSHA Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27958-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

RNASEN, EC:3.1.26.3, ETOHI2, HSA242976, p241

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

27958-1-PBS targets DROSHA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27533

Product name: Recombinant human DROSHA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-550 aa of NM_001100412

Sequence: MHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribonuclease type III, nuclear
别名 RNASEN, EC:3.1.26.3, ETOHI2, HSA242976, p241
计算分子量 159 aa
观测分子量150 kDa
GenBank蛋白编号NM_001100412
基因名称 DROSHA
Gene ID (NCBI) 29102
RRIDAB_2918137
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRR4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA.

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