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验证数据展示

  • WB analysis using 27958-1-AP

    WB analysis using 27958-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27958-1-AP (DROSHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 27958-1-AP

    IF Staining of U2OS using 27958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of HEK-293 using 27958-1-AP

    WB analysis of HEK-293 using 27958-1-AP

    WB result of DROSHA antibody (27958-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DROSHA transfected HEK-293 cells.

  • IF Staining of U2OS using 27958-1-AP

    IF Staining of U2OS using 27958-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

DROSHA Polyclonal antibody

DROSHA Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27958-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RNASEN, EC:3.1.26.3, ETOHI2, HSA242976, p241

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HEK-293 cells, Hela cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27958-1-AP targets DROSHA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 DROSHA fusion protein Ag27533 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribonuclease type III, nuclear
别名 RNASEN, EC:3.1.26.3, ETOHI2, HSA242976, p241
计算分子量 159 aa
观测分子量150 kDa
GenBank蛋白编号NM_001100412
基因名称 DROSHA
Gene ID (NCBI) 29102
RRIDAB_2918137
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRR4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA.

实验方案

Product Specific Protocols
WB protocol for DROSHA antibody 27958-1-APDownload protocol
IF protocol for DROSHA antibody 27958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

N4-acetylcytidine modifies primary microRNAs for processing in cancer cells

Authors - Hailong Zhang
View Article