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验证数据展示

  • WB analysis using 30073-1-AP (same clone as 30073-1-PBS)

    WB analysis using 30073-1-AP (same clone as 30073-1-PBS)

    mouse testis tissue were subjected to Tris-Acetate gel system followed by western blot with 30073-1-AP (DNAH6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 30073-1-AP (same clone as 30073-1-PBS)

    IHC staining of mouse testis using 30073-1-AP (same clone as 30073-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 30073-1-AP (same clone as 30073-1-PBS)

    IHC staining of mouse testis using 30073-1-AP (same clone as 30073-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 30073-1-AP (same clone as 30073-1-PBS)

    IF Staining of hTERT-RPE1 using 30073-1-AP (same clone as 30073-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 30073-1-AP (same clone as 30073-1-PBS)

    IF Staining of hTERT-RPE1 using 30073-1-AP (same clone as 30073-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

DNAH6 Polyclonal antibody, PBS Only

DNAH6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30073-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Ciliary dynein heavy chain 6, Dnahc6, DNHL1, Dynein axonemal heavy chain 6, Dynein heavy chain 6, axonemal

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30073-1-PBS targets DNAH6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32364

Product name: Recombinant human DNAH6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 148-408 aa of BC104884

Sequence: NNKLEGKTCGTGPSLAAVFEDDKNFHTIISQIKETIQAAFESARIYAATFEKFQIFFKENESLDLQALKLQEPDINFFSEQLEKYHKQHKDAVALRPTRNVGLLLIDTRLLREKLIPSPLRCLEVLNFMLPRQSKKKVDAIIFEAQDAEYKLEFVPTTTTEYVHSLLFLDEIQERIESLEDEGNIVTQMYKLMEQYQVPTPPEDFAVFATMKPSIVAVRNAIDKSVGDRESSIKQFCVHLGSDLEELNNEVNEVKLQAQVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, axonemal, heavy chain 6
别名 Ciliary dynein heavy chain 6, Dnahc6, DNHL1, Dynein axonemal heavy chain 6, Dynein heavy chain 6, axonemal
计算分子量475 kDa
观测分子量 475 kDa
GenBank蛋白编号BC104884
基因名称 DNAH6
Gene ID (NCBI) 1768
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0G6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAH6(Dynein heavy chain 6, axonemal) is also named as DNAHC6, DNHL1, HL2, KIAA1697 and belongs to the dynein heavy chain family.Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains(PMID:8812413). It is a force generating protein of respiratory cilia and produces force towards the minus ends of microtubules.This antibody is specific to DNAH6.

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