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验证数据展示

  • WB analysis of SKOV-3 using 15735-1-AP (same clone as 15735-1-PBS)

    WB analysis of SKOV-3 using 15735-1-AP (same clone as 15735-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15735-1-AP (DHRS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15735-1-AP (same clone as 15735-1-PBS)

    IP experiment of HeLa using 15735-1-AP (same clone as 15735-1-PBS)

    IP result of anti-DHRS2 (IP:15735-1-AP, 4ug; Detection:15735-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

    IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DHRS2 antibody included
  • IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

    IHC staining of human liver cancer using 15735-1-AP (same clone as 15735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15735-1-AP (DHRS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DHRS2 antibody included
  • Featured Product
  • KD/KO验证

DHRS2 Polyclonal antibody, PBS Only

DHRS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15735-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DHRS2 Ready-To-Use IHC Kit. DHRS2 primary antibody included.

产品信息

15735-1-PBS targets DHRS2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8508

Product name: Recombinant human DHRS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-271 aa of BC007339

Sequence: QGWFHPCARLSVRMSSTGIDRKGVLANRVAVVTGSTSGIGFAIARRLARDGAHVVISSRKQQNVDRAMAKLQGEGLSVAGIVCHVGKAEDREQLVAKALEHCGGVDFLVCSAGVNPLVGSTLGTSEQIWDKILSVNVKSPALLLSQLLPYMENRRGAVILVSSIAAYNPVVALGVYNVSKTALLGLTRTLALELAPKDIRVNCVVPGIIKTDFSKVFHGNESLWKNFKEHHQLQRIGESEDCAGIVSFLCSPDASYVNGENIAVAGYSTRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dehydrogenase/reductase (SDR family) member 2
别名
计算分子量 300 aa, 32 kDa
观测分子量 27-30 kDa
GenBank蛋白编号BC007339
基因名称 DHRS2
Gene ID (NCBI) 10202
RRIDAB_2091847
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13268
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.

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