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验证数据展示

  • WB analysis using 31363-1-AP (same clone as 31363-1-PBS)

    WB analysis using 31363-1-AP (same clone as 31363-1-PBS)

    Untreated, and etoposide (60uM, 14h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 31363-1-AP (DFNA5/GSDME antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSDME/DFNA5 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 31363-1-AP (same clone as 31363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31363-1-AP (DFNA5 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSDME/DFNA5 antibody included
  • IP experiment of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

    IP experiment of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

    IP result of anti-DFNA5/GSDME (IP:31363-1-AP, 4ug; Detection:31363-1-AP 1:800) with SH-SY5Y cells lysate 6790 ug. This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

    IF Staining of SH-SY5Y using 31363-1-AP (same clone as 31363-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DFNA5 antibody (31363-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31363-1-PBS in a different storage buffer formulation.

DFNA5/GSDME Polyclonal antibody, PBS Only

DFNA5/GSDME Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 31363-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DFNA5, Gasdermin E, Gasdermin-E, GSDME, deafness, autosomal dominant 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.

产品信息

31363-1-PBS targets DFNA5/GSDME in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35186

Product name: Recombinant human DFNA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 90-190 aa of BC019689

Sequence: NVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deafness, autosomal dominant 5
别名 DFNA5, Gasdermin E, Gasdermin-E, GSDME, deafness, autosomal dominant 5
计算分子量 496 aa, 55 kDa
观测分子量55 kDa, 35 kDa, 25 kDa
GenBank蛋白编号BC019689
基因名称 DFNA5
Gene ID (NCBI) 1687
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO60443
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

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