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验证数据展示

  • WB analysis using 21445-1-AP (same clone as 21445-1-PBS)

    WB analysis using 21445-1-AP (same clone as 21445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21445-1-AP (DDRGK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

    IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDRGK1 antibody included
  • IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

    IHC staining of mouse brain using 21445-1-AP (same clone as 21445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21445-1-AP (DDRGK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDRGK1 antibody included
  • IF Staining of HepG2 using 21445-1-AP (same clone as 21445-1-PBS)

    IF Staining of HepG2 using 21445-1-AP (same clone as 21445-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (21445-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21445-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DDRGK1 Polyclonal antibody, PBS Only

DDRGK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21445-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DDRGK1 Ready-To-Use IHC Kit. DDRGK1 primary antibody included.

产品信息

21445-1-PBS targets DDRGK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13742

Product name: Recombinant human DDRGK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-314 aa of BC000643

Sequence: MVAPVWYLVAAALLVGFILFLTRSRGRAASAGQEPLHNEELAGAGRVAQPGPLEPEEPRAGGRPRRRRDLGSRLQAQRRAQRVAWAEADENEEEAVILAQEEEGVEKPAETHLSGKIGAKKLRKLEEKQARKAQREAEEAEREERKRLESQREAEWKKEEERLRLEEEQKEEEERKAREEQAQREHEEYLKLKEAFVVEEEGVGETMTEEQSQSFLTEFINYIKQSKVVLLEDLASQVGLRTQDTINRIQDLLAEGTITGVIDDRGKFIYITPEELAAVANFIRQRGRVSIAELAQASNSLIAWGRESPAQAPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DDRGK domain containing 1
别名
计算分子量 314 aa, 36 kDa
观测分子量 36-46 kDa
GenBank蛋白编号BC000643
基因名称 DDRGK1
Gene ID (NCBI) 65992
RRIDAB_2827383
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HY6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.

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