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验证数据展示

  • WB analysis of mouse liver using 14739-1-AP (same clone as 14739-1-PBS)

    WB analysis of mouse liver using 14739-1-AP (same clone as 14739-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    IHC staining of human liver cancer using 14739-1-AP (same clone as 14739-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14739-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included

CYP27A1 Polyclonal antibody, PBS Only

CYP27A1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14739-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CTX, CP27

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.

产品信息

14739-1-PBS targets CYP27A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6408

Product name: Recombinant human CYP27A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 183-531 aa of BC051851

Sequence: DDFMTRLDQLRAESASGNQVSDMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVLPFWKRYLDGWNAIFSFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQPHRWLRNSQPATPRIQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPETGELKSVARIVLVPNKKVGLQFLQRQC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 27, subfamily A, polypeptide 1
别名 Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CTX, CP27
计算分子量 60 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC051851
基因名称 CYP27A1
Gene ID (NCBI) 1593
RRIDAB_2089276
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02318
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.