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验证数据展示

  • WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

    WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

    WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 28494-1-AP (same clone as 28494-1-PBS)

    IHC staining of human liver cancer using 28494-1-AP (same clone as 28494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 28494-1-AP (same clone as 28494-1-PBS)

    IHC staining of human liver cancer using 28494-1-AP (same clone as 28494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28494-1-AP (same clone as 28494-1-PBS)

    IHC staining of mouse brain using 28494-1-AP (same clone as 28494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28494-1-AP (same clone as 28494-1-PBS)

    IHC staining of mouse brain using 28494-1-AP (same clone as 28494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • IF Staining of COLO 320 using 28494-1-AP (same clone as 28494-1-PBS)

    IF Staining of COLO 320 using 28494-1-AP (same clone as 28494-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CRBN antibody (28494-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CRBN Polyclonal antibody, PBS Only

CRBN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28494-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Protein cereblon, cereblon, AD-006

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

28494-1-PBS targets CRBN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CRBN fusion protein Ag29353 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cereblon
别名 Protein cereblon, cereblon, AD-006
计算分子量 442 aa, 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC017419
基因名称 CRBN
Gene ID (NCBI) 51185
RRIDAB_2881155
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96SW2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).