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验证数据展示

  • IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

    IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COPS6 antibody included
  • WB analysis using 10256-1-AP (same clone as 10256-1-PBS)

    WB analysis using 10256-1-AP (same clone as 10256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • WB analysis of 3T3-L1 using 10256-1-AP (same clone as 10256-1-PBS)

    WB analysis of 3T3-L1 using 10256-1-AP (same clone as 10256-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • IF Staining of COLO 320 using 10256-1-AP (same clone as 10256-1-PBS)

    IF Staining of COLO 320 using 10256-1-AP (same clone as 10256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10256-1-AP (same clone as 10256-1-PBS)

    IF Staining of HeLa using 10256-1-AP (same clone as 10256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

COPS6 Polyclonal antibody, PBS Only

COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10256-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig, monkey

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy COPS6 Ready-To-Use IHC Kit. COPS6 primary antibody included.

产品信息

10256-1-PBS targets COPS6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, monkey
免疫原 COPS6 fusion protein Ag0331 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
别名
计算分子量 327 aa, 36 kDa
观测分子量34 kDa
GenBank蛋白编号BC002520
基因名称 COPS6
Gene ID (NCBI) 10980
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L5N1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.