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验证数据展示

  • WB analysis using 30204-1-AP (same clone as 30204-1-PBS)

    WB analysis using 30204-1-AP (same clone as 30204-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30204-1-AP (COL5A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30204-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 30204-1-AP (same clone as 30204-1-PBS)

    IF Staining of HUVEC using 30204-1-AP (same clone as 30204-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using COL5A3 antibody (30204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30204-1-PBS in a different storage buffer formulation.

COL5A3 Polyclonal antibody, PBS Only

COL5A3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30204-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

COL5, Collagen alpha 3(V) chain, Collagen alpha-3(V) chain, Collagen Type V

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30204-1-PBS targets COL5A3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30705

Product name: Recombinant human COL5A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-380 aa of NM_015719

Sequence: EPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSAENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILERSLDPDSGTELGTLETKAAREDEEGDDSTMGPDFRAAEYPSRTQFQIFPGAGEKGAKGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type V, alpha 3
别名 COL5, Collagen alpha 3(V) chain, Collagen alpha-3(V) chain, Collagen Type V
计算分子量 172 kDa
观测分子量145 kDa
GenBank蛋白编号NM_015719
基因名称 COL5A3
Gene ID (NCBI) 50509
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25940
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COL5A3 (Collagen type V alpha 3) belongs to the fibrillar collagen family. Type V collagen (COLV) is a member of a group I collagen (fibrillar forming collagen) and has three varieties of α chains: α1 (COL5A1), α2 (COL5A2), and α3 (COL5A3). COLV is a minor connective tissue component of nearly ubiquitous distribution. It binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This antibody is specific to COL5A3.

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