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COL5A3 Polyclonal antibody

COL5A3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30204-1-AP

Print datasheet

Synonyms

COL5A3, Collagen alpha 3(V) chain, collagen, type V, alpha 3

验证数据展示

  • WB analysis using 30204-1-AP

    WB analysis using 30204-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30204-1-AP (COL5A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30204-1-AP targets COL5A3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen COL5A3 fusion protein Ag30705 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type V, alpha 3
Synonyms COL5A3, Collagen alpha 3(V) chain, collagen, type V, alpha 3
Calculated Molecular Weight 172 kDa
Observed Molecular Weight145 kDa
GenBank Accession NumberNM_015719
Gene Symbol COL5A3
Gene ID (NCBI) 50509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25940
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL5A3 (Collagen type V alpha 3) belongs to the fibrillar collagen family. Type V collagen (COLV) is a member of a group I collagen (fibrillar forming collagen) and has three varieties of α chains: α1 (COL5A1), α2 (COL5A2), and α3 (COL5A3). COLV is a minor connective tissue component of nearly ubiquitous distribution. It binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This antibody is specific to COL5A3.

实验方案

Product Specific Protocols
WB protocol for COL5A3 antibody 30204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols