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验证数据展示

  • WB analysis using 30204-1-AP

    WB analysis using 30204-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30204-1-AP (COL5A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 30204-1-AP

    IF Staining of HUVEC using 30204-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using COL5A3 antibody (30204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

COL5A3 Polyclonal antibody

COL5A3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30204-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

COL5, Collagen alpha 3(V) chain, Collagen alpha-3(V) chain, Collagen Type V

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells
Positive IF/ICC detected inHUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30204-1-AP targets COL5A3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30705

Product name: Recombinant human COL5A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-380 aa of NM_015719

Sequence: EPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSAENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILERSLDPDSGTELGTLETKAAREDEEGDDSTMGPDFRAAEYPSRTQFQIFPGAGEKGAKGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type V, alpha 3
别名 COL5, Collagen alpha 3(V) chain, Collagen alpha-3(V) chain, Collagen Type V
计算分子量 172 kDa
观测分子量145 kDa
GenBank蛋白编号NM_015719
基因名称 COL5A3
Gene ID (NCBI) 50509
RRIDAB_3669706
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25940
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL5A3 (Collagen type V alpha 3) belongs to the fibrillar collagen family. Type V collagen (COLV) is a member of a group I collagen (fibrillar forming collagen) and has three varieties of α chains: α1 (COL5A1), α2 (COL5A2), and α3 (COL5A3). COLV is a minor connective tissue component of nearly ubiquitous distribution. It binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This antibody is specific to COL5A3.

实验方案

Product Specific Protocols
IF protocol for COL5A3 antibody 30204-1-APDownload protocol
WB protocol for COL5A3 antibody 30204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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