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Species Reactivity
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验证数据展示

  • IHC staining of mouse liver using 30680-1-AP (same clone as 30680-1-PBS)

    IHC staining of mouse liver using 30680-1-AP (same clone as 30680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLOCK antibody included
  • IHC staining of mouse liver using 30680-1-AP (same clone as 30680-1-PBS)

    IHC staining of mouse liver using 30680-1-AP (same clone as 30680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLOCK antibody included
  • IF Staining of HeLa using 30680-1-AP (same clone as 30680-1-PBS)

    IF Staining of HeLa using 30680-1-AP (same clone as 30680-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLOCK antibody (30680-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

CLOCK Polyclonal antibody, PBS Only

CLOCK Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 30680-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CLOCK Ready-To-Use IHC Kit. CLOCK primary antibody included.

产品信息

30680-1-PBS targets CLOCK in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CLOCK fusion protein Ag33370 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clock homolog (mouse)
别名
计算分子量 846 aa, 95 kDa
GenBank蛋白编号BC041878
基因名称 CLOCK
Gene ID (NCBI) 9575
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15516
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. western blot. Our result is similar to the result of reseach paper (PMID: 30683868).