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验证数据展示

  • WB analysis using 28037-1-AP

    WB analysis using 28037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28037-1-AP (CLK3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 28037-1-AP

    IHC staining of human ovary cancer using 28037-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28037-1-AP (CLK3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLK3 antibody included

CLK3 Polyclonal antibody

CLK3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28037-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EC:2.7.12.1, CDC-like kinase 3, CDC like kinase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CLK3 Ready-To-Use IHC Kit. CLK3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28037-1-AP targets CLK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CLK3 fusion protein Ag27741 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CDC-like kinase 3
别名 EC:2.7.12.1, CDC-like kinase 3, CDC like kinase 3
计算分子量 73 kDa
观测分子量56 kDa
GenBank蛋白编号BC002555
基因名称 CLK3
Gene ID (NCBI) 1198
RRIDAB_3669638
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49761
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLK3 (CDC Like Kinase 3) encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that functions on substrates containing serine/threonine and tyrosine. CLK3 modulates RNA splicing by phosphorylating serine/arginine-rich proteins such as SRSF1 and SRSF3. CLK3 dysregulation was indicated to be a high-penetrant factor in different types of human tumors even though its functions in tumors were not clearly characterized (PMID: 32453420). CLK3 has 4 isoforms, which is 74kDa, 59kDa, 56kDa and 18kDa.

实验方案

Product Specific Protocols
WB protocol for CLK3 antibody 28037-1-APDownload protocol
IHC protocol for CLK3 antibody 28037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols