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验证数据展示

  • WB analysis using 31928-1-AP

    WB analysis using 31928-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31928-1-AP (CLEC2L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 31928-1-AP

    IHC staining of mouse skeletal muscle using 31928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31928-1-AP (CLEC2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 31928-1-AP

    IHC staining of mouse cerebellum using 31928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31928-1-AP (CLEC2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CLEC2L Polyclonal antibody

CLEC2L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31928-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

C-Type Lectin Domain Family 2, Member L, C-type lectin domain family 2 member L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse cerebellum tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31928-1-AP targets CLEC2L in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 CLEC2L fusion protein Ag35450 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 C-type lectin domain family 2, member L
别名 C-Type Lectin Domain Family 2, Member L, C-type lectin domain family 2 member L
计算分子量24 kDa
观测分子量38 kDa
GenBank蛋白编号NM_001080511.2
基因名称 CLEC2L
Gene ID (NCBI) 154790
RRIDAB_3670148
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP0C7M8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-type lectin domain family 2 member L (CLEC2L) is a membrane protein mainly expressed in brain tissue and probably has carbohydrate-binding activity. The CLEC2L-encoded C-type lectin-like receptors (CTLRs) are expressed as non-glycosylated, disulfide-linked homodimers at the cell surface, and designated as BACL (brain-associated C-type lectin) (PMID: 23776472). The CLEC2L protein has 214 amino acids with a predicted molecular mass of 23.9 kDa. Under non-reducing conditions, FLAG-tagged CLEC2L proteins appeared with a molecular mass of ~55 kDa in immunoblots because of the occurrence of disulfide-linked homodimers (PMID: 23776472).

实验方案

Product Specific Protocols
WB protocol for CLEC2L antibody 31928-1-APDownload protocol
IHC protocol for CLEC2L antibody 31928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols