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CIDEB Polyclonal antibody

CIDEB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27600-1-AP

Print datasheet

Synonyms

Cell death activator CIDE B, CIDEB

验证数据展示

  • WB analysis using 27600-1-AP

    WB analysis using 27600-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27600-1-AP (CIDEB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27600-1-AP

    IHC staining of mouse liver using 27600-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27600-1-AP targets CIDEB in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen CIDEB fusion protein Ag27003 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell death-inducing DFFA-like effector b
Synonyms Cell death activator CIDE B, CIDEB
Calculated Molecular Weight 219 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC035970
Gene Symbol CIDEB
Gene ID (NCBI) 27141
RRIDAB_2880922
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHD4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cell death‐inducing DFFA‐like effector B (CIDEB), an ER and lipid droplet (LD)‐associated protein primarily expressed in the liver, participated in lipid metabolism by regulating lipid droplet fusion and VLDL lipidation. Although CIDEA is expressed at high levels in brown adipose tissue (BAT) and CIDEC is highly enriched in white adipose tissue (WAT) and BAT, CIDEB is more abundant in liver, kidney, small intestine, and colon. CIDEB is localized to endoplasmic reticulum and lipid droplets. (PMID: 30858281, PMID: 24831470, PMID: 19187774)

实验方案

Product Specific Protocols
WB protocol for CIDEB antibody 27600-1-APDownload protocol
IHC protocol for CIDEB antibody 27600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Hazard Mater

TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.

Authors - Changying Li
View Article
humanWB

Cancer Biol Ther

The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer

Authors - Wen-Jing Yun
View Article