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验证数据展示

  • WB analysis using 13402-1-AP (same clone as 13402-1-PBS)

    WB analysis using 13402-1-AP (same clone as 13402-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

    IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHD9 antibody included
  • IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

    IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHD9 antibody included
  • IF Staining of Hela using 13402-1-AP (same clone as 13402-1-PBS)

    IF Staining of Hela using 13402-1-AP (same clone as 13402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CHD9 antibody (13402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CHD9 Polyclonal antibody, PBS Only

CHD9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13402-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AD013, AD-013, ATP-dependent helicase CHD9, CHD-9, Chromatin-related mesenchymal modulator

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHD9 Ready-To-Use IHC Kit. CHD9 primary antibody included.

产品信息

13402-1-PBS targets CHD9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4245

Product name: Recombinant human CHD9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC033770

Sequence: MTDPMMDFFDDANLFGETLEGLSDDAFVQPGPVSLVDELNLGAEFEPLHIDSLNHVQGTPTHQKMTDFEQLNQFDSIKFHHVNQSFGSPAEHVLSPHSQFNCSPIHPQNQPNGLFPDVSDGSPMWGHQTATTISNQNGSPFHQQGHSHSMHQNKSFVAHHDFALFQANEQQTQCTSLRSQQNRNNLNPGQNSLSQSKNFMNVSGPHRVNVNHPPQMTNASNSQQSISMQQFSQTSNPSAHFHKCSSHQEGNFNGPSPNMTSCSVSNSQQFSSHYSFSSNHISPNSLLQSSAVLASNHTNQTLSDFTGSNSFSPHRGIKQESTQHILNPNTSLNSNNFQILHSSHPQGNYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromodomain helicase DNA binding protein 9
别名 AD013, AD-013, ATP-dependent helicase CHD9, CHD-9, Chromatin-related mesenchymal modulator
计算分子量 2897 aa, 326 kDa
观测分子量 330 kDa
GenBank蛋白编号BC033770
基因名称 CHD9
Gene ID (NCBI) 80205
RRIDAB_10640425
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3L8U1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHD (chromodomain helicase DNA-binding) proteins are chromatin remodelling enzymes which reshape the local chromatin architecture to allow access to transcriptional machinery in a tissue-specific context. CHD proteins facilitate various stages of gene expression, including binding and recruiting transcription factors, histone modifications, and transcriptional elongation, termination and processing. CHD proteins are characterized by two tandem chromodomains and a helicase C domain, but also possesses additional functional domains which classify them into three subfamilies. CHD9 is active in mesenchymal stromal stem cells and osteoprogenitor cells, and is suggested to control osteogenic cell fate (PMID: 32295522).

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