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验证数据展示

  • WB analysis of HeLa using 25612-1-AP (same clone as 25612-1-PBS)

    WB analysis of HeLa using 25612-1-AP (same clone as 25612-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 25612-1-AP (same clone as 25612-1-PBS)

    IF Staining of HeLa using 25612-1-AP (same clone as 25612-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25612-1-AP (CEBPZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • WB analysis using 25612-1-AP (same clone as 25612-1-PBS)

    WB analysis using 25612-1-AP (same clone as 25612-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

  • WB analysis using 25612-1-AP (same clone as 25612-1-PBS)

    WB analysis using 25612-1-AP (same clone as 25612-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25612-1-PBS in a different storage buffer formulation.

CEBPZ Polyclonal antibody, PBS Only

CEBPZ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25612-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25612-1-PBS targets CEBPZ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11003

Product name: Recombinant human CEBPZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 705-1054 aa of BC034475

Sequence: FCGAENTSLWELKKLSVHFHPSVALFAKTILQGNYIQYSGDPLQDFTLMRFLDRFVYRNPKPHKGKENTDSVVMQPKRKHFIKDIRHLPVNSKEFLAKEESQIPVDEVFFHRYYKKVAVKEKQKRDADEESIEDVDDEEFEELIDTFEDDNCFSSGKDDMDFAGNVKKRTKGAKDNTLDEDSEGSDDELGNLDDDEVSLGSMDDEEFAEVDEDGGTFMDVLDDESESVPELEVHSKVSTKKSKRKGTDDFDFAGSFQGPRKKKRNLNDSSLFVSAEEFGHLLDENMGSKFDNIGMNAMANKDNASLKQLRWEAERDDWLHNRDAKSIIKKKKHFKKKRIKTTQKTKKQRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CCAAT/enhancer binding protein (C/EBP), zeta
别名
计算分子量 1054 aa, 121 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC034475
基因名称 CEBPZ
Gene ID (NCBI) 10153
RRIDAB_2880160
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03701
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEBPZ (CCAAT/enhancer binding protein zeta) belongs to the CBF/Mak21 family, it plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner.

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