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验证数据展示

  • FC experiment of K-562 using CL647-67239

    FC experiment of K-562 using CL647-67239

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human CDC27; APC3 (CL647-67239, Clone:2F10G2) (red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11) (CL647-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 647-conjugated CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for FC (Intra)
Cat No. CL647-67239

产品说明书

CloneNo. 2F10G2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

FC (Intra)

别名

ANAPC3, APC3, CDC27, CDC27 homolog, CDC27; APC3, CDC27Hs, D0S1430E, D17S978E, H NUC, HNUC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy CDC27 Ready-To-Use IHC Kit. CDC27 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67239-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67239 targets CDC27; APC3 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat
免疫原 CDC27; APC3 fusion protein Ag25588 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cell division cycle 27 homolog (S. cerevisiae)
别名 ANAPC3, APC3, CDC27, CDC27 homolog, CDC27; APC3, CDC27Hs, D0S1430E, D17S978E, H NUC, HNUC
计算分子量 92 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC011656
基因名称 CDC27
Gene ID (NCBI) 996
RRIDAB_2935075
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30260
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.