Validation Data Gallery
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
产品信息
CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).
| Source | CHO cells-derived Human CD19 protein (20-291 aa) (Accession #P15391) with a mutated Human IgG1 FcZero backbone |
| Isotype | Recombinant Human IgG1 |
| Tested Reactivity | Human |
| Applications | FC |
| Target Cell | CD19-specific CAR-T cells, CD19-specific CAR-NK cells |
| Backbone | Human IgG1 FcZero backbone |
| Site Specific Conjugation | Site specific conjugated with PE |
| Form | Liquid |
| GeneID | 930 |
| UNIPROT ID | P15391 |
| Conjugate | PE |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Storage Buffer | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
