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CoraLite® Plus 488-conjugated CASPR Polyclonal antibody

CASPR Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-55417

Print datasheet

Synonyms

CASPR, Caspr1, CNTNAP, CNTNAP1, contactin associated protein 1, Neurexin 4, Neurexin IV, NRXN4, P190

验证数据展示

  • FC experiment of SH-SY5Y using CL488-55417

    FC experiment of SH-SY5Y using CL488-55417

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CASPR (CL488-55417) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 55417-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-55417 targets CASPR in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name contactin associated protein 1
Synonyms CASPR, Caspr1, CNTNAP, CNTNAP1, contactin associated protein 1, Neurexin 4, Neurexin IV, NRXN4, P190
Calculated Molecular Weight 156 kDa
Observed Molecular Weight 180-190 kDa
GenBank Accession NumberNM_003632
Gene Symbol CNTNAP1
Gene ID (NCBI) 8506
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDP78357
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNTNAP1, also named as CASPR, NRXN4, p190, Neurexin IV and Neurexin-4, belongs to the neurexin family. It plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. CNTNAP1 seems to demarcate the paranodal region of the axo-glial junction. In association with contactin, CNTNAP1 may have a role in the signaling between axons and myelinating glial cells. This antibody is specific to CNTNAP1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CASPR antibody CL488-55417Download protocol
Standard Protocols
Click here to view our Standard Protocols