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CoraLite® Plus 488-conjugated CAPZB Monoclonal antibody

CAPZB Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F9G2

Cat No : CL488-68190

Print datasheet

Synonyms

CAPB, CAPPB, CAPZ, CapZ beta, CAPZB

验证数据展示

  • FC experiment of Jurkat using CL488-68190

    FC experiment of Jurkat using CL488-68190

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CAPZB (CL488-68190, Clone:1F9G2) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using CL488-68190

    IF Staining of A431 using CL488-68190

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 CAPZB antibody (CL488-68190, Clone: 1F9G2 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inA431 cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CAPZB Ready-To-Use IHC Kit. CAPZB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68190-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68190 targets CAPZB in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen CAPZB fusion protein Ag19373 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name capping protein (actin filament) muscle Z-line, beta
Synonyms CAPB, CAPPB, CAPZ, CapZ beta, CAPZB
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC109241
Gene Symbol CAPZB
Gene ID (NCBI) 832
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47756
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CAPZB antibody CL488-68190Download protocol
FC protocol for CL Plus 488 CAPZB antibody CL488-68190Download protocol
Standard Protocols
Click here to view our Standard Protocols