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验证数据展示

  • WB analysis of human blood using 17239-1-AP (same clone as 17239-1-PBS)

    WB analysis of human blood using 17239-1-AP (same clone as 17239-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 17239-1-AP (C6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 17239-1-AP (same clone as 17239-1-PBS)

    IHC staining of mouse liver using 17239-1-AP (same clone as 17239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 17239-1-AP (same clone as 17239-1-PBS)

    IHC staining of mouse liver using 17239-1-AP (same clone as 17239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 17239-1-AP (same clone as 17239-1-PBS)

    IP experiment of human plasma using 17239-1-AP (same clone as 17239-1-PBS)

    IP result of anti-Complement C6 (IP:17239-1-AP, 4ug; Detection:17239-1-AP 1:3000) with human plasma lysate 3200 ug. This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

Complement C6 Polyclonal antibody, PBS Only

Complement C6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17239-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

C6, complement component 6, Complement component C6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

17239-1-PBS targets Complement C6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11083

Product name: Recombinant human C6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 586-934 aa of BC035723

Sequence: TRECNNPAPQRGGKRCEGEKRQEEDCTFSIMENNGQPCINDDEEMKEVDLPEIEADSGCPQPVPPENGFIRNEKQLYLVGEDVEISCLTGFETVGYQYFRCLPDGTWRQGDVECQRTECIKPVVQEVLTITPFQRLYRIGESIELTCPKGFVVAGPSRYTCQGNSWTPPISNSLTCEKDTLTKLKGHCQLGQKQSGSECICMSPEEDCSHHSEDLCVFDTDSNDYFTSPACKFLAEKCLNNQQLHFLHIGSCQDGRQLEWGLERTRLSSNSTKKESCGYDTCYDWEKCSASTSKCVCLLPPQCFKGGNQLYCVKMGSSTSEKTLNICEVGTIRCANRKMEILHPGKCLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 6
别名 C6, complement component 6, Complement component C6
计算分子量 934 aa, 105 kDa
观测分子量105-120 kDa
GenBank蛋白编号BC035723
基因名称 C6
Gene ID (NCBI) 729
RRIDAB_2878366
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13671
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The final consequence of complement activation is formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC. The deduced C6 protein contains 934 amino acids, including a 21-amino acid N-terminal signal sequence and 2 N-glycosylation sites. Mutations in the C6 gene are associated with complement component-6 deficiency.

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