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验证数据展示

  • WB analysis using 24820-1-AP (same clone as 24820-1-PBS)

    WB analysis using 24820-1-AP (same clone as 24820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24820-1-PBS in a different storage buffer formulation.

  • WB analysis using 24820-1-AP (same clone as 24820-1-PBS)

    WB analysis using 24820-1-AP (same clone as 24820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24820-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 24820-1-AP (same clone as 24820-1-PBS)

    IF Staining of U2OS using 24820-1-AP (same clone as 24820-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C1RL antibody (24820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24820-1-PBS in a different storage buffer formulation.

C1RL Polyclonal antibody, PBS Only

C1RL Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24820-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

C1r like protein, C1r LP, C1RL1, C1r-like protein, C1r-like serine protease analog protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

24820-1-PBS targets C1RL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20324

Product name: Recombinant human C1RL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 41-195 aa of BC156780

Sequence: SVLLAQELPQQLTSPGYPEPYGKGQESSTDIKAPEGFAVRLVFQDFDLEPSQDCAGDSVTISFVGSDPSQFCGQQGSPLGRPPGQREFVSSGRSLRLTFRTQPSSENKTAHLHKGFLALYQTVAVNYSQPISEASRGSEAINAPGDNPAKVQNHC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 1, r subcomponent-like
别名 C1r like protein, C1r LP, C1RL1, C1r-like protein, C1r-like serine protease analog protein
计算分子量 487 aa, 53 kDa
观测分子量53 kDa, 70 kDa
GenBank蛋白编号BC156780
基因名称 C1RL
Gene ID (NCBI) 51279
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZP8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C1r-like serine protease analog protein (CLSPa), also named as CIRL contains a CUB domain and a serine protease domain, possessing characteristic catalytic triad. C1RL is upregulated in glioblastoma (GBM) and has been proved to be a prognostic marker in hepatocellular carcinoma and renal cell cancer (PMID: 32993564). C1RL has a calculated molecular weight of 53 kDa, and this antibody can detect a band at 70 kDa due to possible N-glycosylation (PMID: 15358180).

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