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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30212-1-AP (same clone as 30212-1-PBS)

    WB analysis using 30212-1-AP (same clone as 30212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30212-1-AP (BRAWNIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30212-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 30212-1-AP (same clone as 30212-1-PBS)

    IF Staining of U-251 using 30212-1-AP (same clone as 30212-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30212-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 30212-1-AP (same clone as 30212-1-PBS)

    IF Staining of U-251 using 30212-1-AP (same clone as 30212-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30212-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 30212-1-AP (same clone as 30212-1-PBS)

    IF Staining of A431 using 30212-1-AP (same clone as 30212-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30212-1-PBS in a different storage buffer formulation.

BRAWNIN Polyclonal antibody, PBS Only

BRAWNIN Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30212-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30212-1-PBS targets BRAWNIN in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 BRAWNIN fusion protein Ag32955 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 12 open reading frame 73
别名
计算分子量8 kDa
观测分子量 9 kDa
基因名称 C12orf73
Gene ID (NCBI) 728568
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ69YU5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquinol-cytochrome-c reductase complex assembly factor 6 (UQCC6) is also named as BRAWNIN, BR and C12orf73, and belongs to the UQCC6 family. BRAWNIN (BR), a 71 a.a. peptide encoded by C12orf73, is essential for respiratory chain complex III (CIII) assembly. In human cells, BRAWNIN is induced by the energy-sensing AMPK pathway, and its depletion impairs mitochondrial ATP production. In zebrafish, Brawnin deletion causes complete CIII loss, resulting in severe growth retardation, lactic acidosis and early death (PMID:32161263).  BR is also detectable in human cardiac and skeletal muscle where it displays a staining pattern characteristic of the mitochondria network. BR protein abundance in mouse tissues correlated well with that of mitochondrial respiratory chain proteins, being high in brown adipose, cardiac and skeletal muscle but virtually undetectable in white adipose tissue (PMID:32161263). BR resides in the IMM and not in the outer mitochondrial membrane (OMM) (PMID:32161263).