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验证数据展示

  • WB analysis using 30212-1-AP

    WB analysis using 30212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30212-1-AP (BRAWNIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 30212-1-AP

    IF Staining of U-251 using 30212-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U-251 using 30212-1-AP

    IF Staining of U-251 using 30212-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A431 using 30212-1-AP

    IF Staining of A431 using 30212-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

BRAWNIN Polyclonal antibody

BRAWNIN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30212-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

BR, BRAWNIN, C12orf73, DKFZp547P055, FLJ13975, UQCC6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IF/ICC detected inU-251 cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:400-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30212-1-AP targets BRAWNIN in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 BRAWNIN fusion protein Ag32955 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 12 open reading frame 73
别名 BR, BRAWNIN, C12orf73, DKFZp547P055, FLJ13975, UQCC6
计算分子量8 kDa
观测分子量 9 kDa
基因名称 C12orf73
Gene ID (NCBI) 728568
RRIDAB_3086265
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ69YU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquinol-cytochrome-c reductase complex assembly factor 6 (UQCC6) is also named as BRAWNIN, BR and C12orf73, and belongs to the UQCC6 family. BRAWNIN (BR), a 71 a.a. peptide encoded by C12orf73, is essential for respiratory chain complex III (CIII) assembly. In human cells, BRAWNIN is induced by the energy-sensing AMPK pathway, and its depletion impairs mitochondrial ATP production. In zebrafish, Brawnin deletion causes complete CIII loss, resulting in severe growth retardation, lactic acidosis and early death (PMID:32161263).  BR is also detectable in human cardiac and skeletal muscle where it displays a staining pattern characteristic of the mitochondria network. BR protein abundance in mouse tissues correlated well with that of mitochondrial respiratory chain proteins, being high in brown adipose, cardiac and skeletal muscle but virtually undetectable in white adipose tissue (PMID:32161263). BR resides in the IMM and not in the outer mitochondrial membrane (OMM) (PMID:32161263).

实验方案

Product Specific Protocols
WB protocol for BRAWNIN antibody 30212-1-APDownload protocol
IF protocol for BRAWNIN antibody 30212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols