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验证数据展示

  • WB analysis of K-562 using 16354-1-AP (same clone as 16354-1-PBS)

    WB analysis of K-562 using 16354-1-AP (same clone as 16354-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 16354-1-AP (same clone as 16354-1-PBS)

    WB analysis of Jurkat using 16354-1-AP (same clone as 16354-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 16354-1-AP (same clone as 16354-1-PBS)

    IHC staining of human liver cancer using 16354-1-AP (same clone as 16354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 16354-1-AP (same clone as 16354-1-PBS)

    IHC staining of human liver cancer using 16354-1-AP (same clone as 16354-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16354-1-PBS in a different storage buffer formulation.

BLZF1 Polyclonal antibody, PBS Only

BLZF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16354-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16354-1-PBS targets BLZF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9564

Product name: Recombinant human BLZF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 48-400 aa of BC020716

Sequence: QSPWKKAVPSESPGVLQLGKMLTEKAMEVKAVRILVPKAAITHDIPNKNTKVKSLGHHKGEFLGQSEGVIEPNKELSEVKNVLEKLKNSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNQLILENEALGRNTAQLSEQLERMSIQCDVWRSKFLASRVMADELTNSRAALQRQNRDAHGAIQDLLSEREQFRQEMIATQKLLEELLVSLQWGREQTYSPSVQPHSTAELALTNHKLAKAVNSHLLGNVGINNQKKIPSTVEFCSTPAEKMAETVLRILDPVTCKESSPDNPFFESSPTTLLATKKNIGRFHPYTRYENITFNCCNHCRGELIAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basic leucine zipper nuclear factor 1
别名
计算分子量 400 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC020716
基因名称 BLZF1
Gene ID (NCBI) 8548
RRIDAB_2918045
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2G9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BLZF1 (basic leucine zipper nuclear factor 1), also known as JEM1 or Golgin-45, maps to human chromosome 1q24, encodes for a 3 kb mRNA that is translated into a 45 kDa protein. BLZF1 is a member of b-ZIP family, and has been implicated in epigenetic regulation and Wnt/β-catenin signaling.