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验证数据展示

  • WB analysis of PC-12 using 81976-3-RR (same clone as 81976-3-PBS)

    WB analysis of PC-12 using 81976-3-RR (same clone as 81976-3-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81976-3-PBS in a different storage buffer formulation.

  • WB analysis of mouse thymus using 81976-3-RR (same clone as 81976-3-PBS)

    WB analysis of mouse thymus using 81976-3-RR (same clone as 81976-3-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81976-3-PBS in a different storage buffer formulation.

  • WB analysis of A20 using 81976-3-RR (same clone as 81976-3-PBS)

    WB analysis of A20 using 81976-3-RR (same clone as 81976-3-PBS)

    A20 cells were subjected to SDS PAGE followed by western blot with 81976-3-RR (BIM, BCL2L11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81976-3-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02863-1

    Cytometric bead array sample test of MP02863-1

    The mean BIM/BCL2L11 concentration was determined to be 4.4 ng/mL in Raji cell extract based on a 1.3 mg/mL extract load, 1.7 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02863-1

    Cytometric bead array standard curve of MP02863-1

    Cytometric bead array standard curve of MP02863-1, BIM/BCL2L11 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81976-3-PBS. Detection antibody: 81976-2-PBS. Standard: Ag11875. Range: 0.156-20 ng/mL.

  • Affinity of 81976-3-RR

    Affinity of 81976-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81976-3-RR against Human BIM/BCL2L11 were performed. The affinity constant is below 1 pM.

BIM/BCL2L11 Recombinant monoclonal antibody, PBS Only (Capture)

BIM/BCL2L11 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 81976-3-PBS

产品说明书

CloneNo. 252250A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

BCL2L11, BAM, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2L11, BIM

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BCL2L11/BIM Ready-To-Use IHC Kit. BCL2L11/BIM primary antibody included.

产品信息

81976-3-PBS targets BIM/BCL2L11 as part of a matched antibody pair:

MP02863-1: 81976-3-PBS capture and 81976-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11875

Product name: Recombinant human BIM, BCL2L11 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-198 aa of BC033694

Sequence: MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-like 11 (apoptosis facilitator)
别名 BCL2L11, BAM, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2L11, BIM
计算分子量 198 aa, 22 kDa
观测分子量16, 23 kDa
GenBank蛋白编号BC033694
基因名称 Bim
Gene ID (NCBI) 10018
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43521
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BIM (BCL2L11) is a central BH3-only apoptosis activator that antagonizes anti-apoptotic BCL-2 proteins and directly triggers BAX/BAK-mediated mitochondrial permeabilization. Induced by cellular stress and cytokine withdrawal, BIM governs developmental cell death, immune tolerance, and tumor suppression. Alterations in BIM expression or splicing-particularly the deletion polymorphism affecting its BH3-only activity-underlie cancer therapy resistance, autoimmune disorders, and neuronal apoptosis.

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