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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
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验证数据展示

  • WB analysis using 28622-1-AP (same clone as 28622-1-PBS)

    WB analysis using 28622-1-AP (same clone as 28622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28622-1-AP (BCAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 28622-1-AP (same clone as 28622-1-PBS)

    IHC staining of human liver cancer using 28622-1-AP (same clone as 28622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

BCAT1 Polyclonal antibody, PBS Only

BCAT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28622-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

28622-1-PBS targets BCAT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag28998

Product name: Recombinant human BCAT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-115 aa of BC033864

Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 branched chain aminotransferase 1, cytosolic
别名
计算分子量 320 aa, 36 kDa
观测分子量 43-48 kDa
GenBank蛋白编号BC033864
基因名称 BCAT1
Gene ID (NCBI) 586
RRIDAB_2881183
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54687
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

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