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Host
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Species Reactivity
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验证数据展示

  • WB analysis using 30505-1-AP (same clone as 30505-1-PBS)

    WB analysis using 30505-1-AP (same clone as 30505-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30505-1-PBS in a different storage buffer formulation.

  • WB analysis using 30505-1-AP (same clone as 30505-1-PBS)

    WB analysis using 30505-1-AP (same clone as 30505-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30505-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 30505-1-AP (same clone as 30505-1-PBS)

    IHC staining of human colon cancer using 30505-1-AP (same clone as 30505-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30505-1-AP (ATG12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30505-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 30505-1-AP (same clone as 30505-1-PBS)

    IF Staining of PC-3 using 30505-1-AP (same clone as 30505-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ATG12 antibody (30505-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30505-1-PBS in a different storage buffer formulation.

ATG12 Polyclonal antibody, PBS Only

ATG12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30505-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

APG12, APG12 like, APG12L, APG12-like, Autophagy-related protein 12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

30505-1-PBS targets ATG12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ATG12 fusion protein Ag32268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATG12 autophagy related 12 homolog (S. cerevisiae)
别名 APG12, APG12 like, APG12L, APG12-like, Autophagy-related protein 12
计算分子量 15 kDa
观测分子量20 kDa, 50-60 kDa
GenBank蛋白编号BC012266
基因名称 ATG12
Gene ID (NCBI) 9140
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94817
储存缓冲液 PBS only{{ptg:BufferTemp}}7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.