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验证数据展示

  • WB analysis using 16882-1-AP

    WB analysis using 16882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16882-1-AP (ATAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 16882-1-AP

    IP experiment of mouse brain using 16882-1-AP

    IP result of anti-ATAD1 (IP:16882-1-AP, 4ug; Detection:16882-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

  • IHC staining of human colon cancer using 16882-1-AP

    IHC staining of human colon cancer using 16882-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16882-1-AP (ATAD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATAD1 Polyclonal antibody

ATAD1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16882-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

hATAD1, FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16882-1-AP targets ATAD1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10343

Product name: Recombinant human ATAD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 164-361 aa of BC010868

Sequence: DKWYGESQKLAAAVFSLAIKLQPSIIFIDEIDSFLRNRSSSDHEATAMMKAQFMSLWDGLDTDHSCQVIVMGATNRPQDLDSAIMRRMPTRFHINQPALKQREAILKLILKNENVDRHVDLLEVAQETDGFSGSDLKEMCRDAALLCVREYVNSTSEESHDEDEIRPVQQQDLHRAIEKMKKSKDAAFQNVLTHVCLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATPase family, AAA domain containing 1
别名 hATAD1, FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1
计算分子量 361 aa, 41 kDa
观测分子量 32 kDa
GenBank蛋白编号BC010868
基因名称 ATAD1
Gene ID (NCBI) 84896
RRIDAB_2878327
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NBU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.

实验方案

Product Specific Protocols
WB protocol for ATAD1 antibody 16882-1-APDownload protocol
IHC protocol for ATAD1 antibody 16882-1-APDownload protocol
IP protocol for ATAD1 antibody 16882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols