• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATAD1 Polyclonal antibody

ATAD1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16882-1-AP

Print datasheet

Synonyms

AFDC1, ATAD1, FNP001

验证数据展示

  • WB analysis using 16882-1-AP

    WB analysis using 16882-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16882-1-AP (ATAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 16882-1-AP

    IP experiment of mouse brain using 16882-1-AP

    IP result of anti-ATAD1 (IP:16882-1-AP, 4ug; Detection:16882-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

经过测试的应用

Positive WB detected inHepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16882-1-AP targets ATAD1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ATAD1 fusion protein Ag10343 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase family, AAA domain containing 1
Synonyms AFDC1, ATAD1, FNP001
Calculated Molecular Weight 361 aa, 41 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC010868
Gene Symbol ATAD1
Gene ID (NCBI) 84896
RRIDAB_2878327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.

实验方案

Product Specific Protocols
WB protocol for ATAD1 antibody 16882-1-APDownload protocol
IP protocol for ATAD1 antibody 16882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols