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Species Reactivity
0
Species Reactivity
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Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 30467-1-AP (same clone as 30467-1-PBS)

    WB analysis using 30467-1-AP (same clone as 30467-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30467-1-AP (ANKIB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 30467-1-AP (same clone as 30467-1-PBS)

    IHC staining of mouse kidney using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 30467-1-AP (same clone as 30467-1-PBS)

    IHC staining of mouse kidney using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse placenta using 30467-1-AP (same clone as 30467-1-PBS)

    IHC staining of mouse placenta using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse placenta using 30467-1-AP (same clone as 30467-1-PBS)

    IHC staining of mouse placenta using 30467-1-AP (same clone as 30467-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 30467-1-AP (same clone as 30467-1-PBS)

    IP experiment of HeLa using 30467-1-AP (same clone as 30467-1-PBS)

    IP result of anti-ANKIB1 (IP:30467-1-AP, 4ug; Detection:30467-1-AP 1:4000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30467-1-AP (same clone as 30467-1-PBS)

    IF Staining of U2OS using 30467-1-AP (same clone as 30467-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 30467-1-AP (same clone as 30467-1-PBS)

    IF Staining of A431 using 30467-1-AP (same clone as 30467-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30467-1-PBS in a different storage buffer formulation.

ANKIB1 Polyclonal antibody, PBS Only

ANKIB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 30467-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

30467-1-PBS targets ANKIB1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ANKIB1 fusion protein Ag33126 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat and IBR domain containing 1
别名
计算分子量122 kDa
观测分子量 122 kDa
GenBank蛋白编号NM_019004.1
基因名称 ANKIB1
Gene ID (NCBI) 54467
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2G1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ankyrin repeat and IBR domain-containing protein 1 (ANKIB1) is also named as KIAA1386, and belongs to the RBR family. ANKIB1 might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. The common biomarkers found within the H and EC region of brain are ZNF621, SLC25A46, RAE1, and ANKIB1. Among these biomarkers, RAE1, ANKIB1, and SLC25A46 have been reported to be prominently involved in several neurodegenerative disorders (PMID:33878365). ANKIB1 is also found to be associated with Cerebral cavernous malformations (PMID:20798775).