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验证数据展示

  • WB analysis of mouse small intestine using 86505-1-RR

    WB analysis of mouse small intestine using 86505-1-RR

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 86505-1-RR (ALPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat small intestine using 86505-1-RR

    WB analysis of rat small intestine using 86505-1-RR

    rat small intestine tissue were subjected to SDS PAGE followed by western blot with 86505-1-RR (ALPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ALPI Recombinant monoclonal antibody

ALPI Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86505-1-RR

产品说明书

CloneNo. 251267F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

EC:3.1.3.1, IAP, Intestinal alkaline phosphatase, Intestinal-type alkaline phosphatase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse small intestine tissue, rat small intestine tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86505-1-RR targets ALPI in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg3033

Product name: Recombinant Human ALPI protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-528 aa of NM_001631.5

Sequence: VIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTTDAAHPVAASLPLLAGTLLLLGASAAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alkaline phosphatase, intestinal
别名 EC:3.1.3.1, IAP, Intestinal alkaline phosphatase, Intestinal-type alkaline phosphatase
计算分子量57 kDa
观测分子量70-75 kDa
GenBank蛋白编号NM_001631.5
基因名称 ALPI
Gene ID (NCBI) 248
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09923
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intestinal-type alkaline phosphatase (IAP) is one of the four human alkaline-phosphatase isoenzymes. It is synthesized by enterocytes of the small-intestinal brush border and is released into the gut lumen, blood and stool. Intestinal-type alkaline phosphatase is a constitutive, evolutionarily conserved sentinel that chemically disarms microbial toxins, orchestrates innate immunity and preserves gut homeostasis.

实验方案

Product Specific Protocols
WB protocol for ALPI antibody 86505-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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