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验证数据展示

  • WB analysis of mouse small intestine using 86505-1-RR (same clone as 86505-1-PBS)

    WB analysis of mouse small intestine using 86505-1-RR (same clone as 86505-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 86505-1-RR (ALPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86505-1-PBS in a different storage buffer formulation.

  • WB analysis of rat small intestine using 86505-1-RR (same clone as 86505-1-PBS)

    WB analysis of rat small intestine using 86505-1-RR (same clone as 86505-1-PBS)

    rat small intestine tissue were subjected to SDS PAGE followed by western blot with 86505-1-RR (ALPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86505-1-PBS in a different storage buffer formulation.

ALPI Recombinant monoclonal antibody, PBS Only

ALPI Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86505-1-PBS

产品说明书

CloneNo. 251267F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

EC:3.1.3.1, IAP, Intestinal alkaline phosphatase, Intestinal-type alkaline phosphatase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86505-1-PBS targets ALPI in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg3033

Product name: Recombinant Human ALPI protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-528 aa of NM_001631.5

Sequence: VIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTTDAAHPVAASLPLLAGTLLLLGASAAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alkaline phosphatase, intestinal
别名 EC:3.1.3.1, IAP, Intestinal alkaline phosphatase, Intestinal-type alkaline phosphatase
计算分子量57 kDa
观测分子量70-75 kDa
GenBank蛋白编号NM_001631.5
基因名称 ALPI
Gene ID (NCBI) 248
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09923
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Intestinal-type alkaline phosphatase (IAP) is one of the four human alkaline-phosphatase isoenzymes. It is synthesized by enterocytes of the small-intestinal brush border and is released into the gut lumen, blood and stool. Intestinal-type alkaline phosphatase is a constitutive, evolutionarily conserved sentinel that chemically disarms microbial toxins, orchestrates innate immunity and preserves gut homeostasis.

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