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验证数据展示

  • WB analysis of HeLa using 22220-1-AP (same clone as 22220-1-PBS)

    WB analysis of HeLa using 22220-1-AP (same clone as 22220-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 22220-1-AP (same clone as 22220-1-PBS)

    IHC staining of human liver using 22220-1-AP (same clone as 22220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of rat kidney using 22220-1-AP (same clone as 22220-1-PBS)

    IHC staining of rat kidney using 22220-1-AP (same clone as 22220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of rat kidney using 22220-1-AP (same clone as 22220-1-PBS)

    IHC staining of rat kidney using 22220-1-AP (same clone as 22220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of human colon cancer using 22220-1-AP (same clone as 22220-1-PBS)

    IHC staining of human colon cancer using 22220-1-AP (same clone as 22220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of human colon cancer using 22220-1-AP (same clone as 22220-1-PBS)

    IHC staining of human colon cancer using 22220-1-AP (same clone as 22220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of human ovary tumor using 22220-1-AP (same clone as 22220-1-PBS)

    IHC staining of human ovary tumor using 22220-1-AP (same clone as 22220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of human liver using 22220-1-AP (same clone as 22220-1-PBS)

    IHC staining of human liver using 22220-1-AP (same clone as 22220-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IF Staining of MCF-7 using 22220-1-AP (same clone as 22220-1-PBS)

    IF Staining of MCF-7 using 22220-1-AP (same clone as 22220-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ALDH1B1 antibody (22220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 22220-1-AP (same clone as 22220-1-PBS)

    IF Staining of HepG2 using 22220-1-AP (same clone as 22220-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDH1B1 antibody (22220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 22220-1-PBS in a different storage buffer formulation.

ALDH1B1 Polyclonal antibody, PBS Only

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22220-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX, EC:1.2.1.3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALDH1B1 Ready-To-Use IHC Kit. ALDH1B1 primary antibody included.

产品信息

22220-1-PBS targets ALDH1B1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17557

Product name: Recombinant human ALDH1B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 330-382 aa of BC001619

Sequence: SIYNEFLERTVEKAKQRKVGNPFELDTQQGPQVDKEQFERVLGYIQLGQKEGA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 1 family, member B1
别名 Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX, EC:1.2.1.3
计算分子量 517 aa, 57 kDa
观测分子量 55-58 kDa
GenBank蛋白编号BC001619
基因名称 ALDH1B1
Gene ID (NCBI) 219
RRIDAB_2879035
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30837
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

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