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验证数据展示

  • IHC staining of mouse heart using 14978-1-AP (same clone as 14978-1-PBS)

    IHC staining of mouse heart using 14978-1-AP (same clone as 14978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AK1 antibody included
  • IHC staining of mouse heart using 14978-1-AP (same clone as 14978-1-PBS)

    IHC staining of mouse heart using 14978-1-AP (same clone as 14978-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14978-1-AP (AK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AK1 antibody included
  • WB analysis using 14978-1-AP (same clone as 14978-1-PBS)

    WB analysis using 14978-1-AP (same clone as 14978-1-PBS)

    mouse heart lysates were subjected to SDS PAGE followed by western blot with 14978-1-AP (AK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14978-1-PBS in a different storage buffer formulation.

AK1 Polyclonal antibody, PBS Only

AK1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14978-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

adenylate kinase 1, Adenylate kinase isoenzyme 1, Adenylate monophosphate kinase, AK 1, ATP:AMP phosphotransferase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AK1 Ready-To-Use IHC Kit. AK1 primary antibody included.

产品信息

14978-1-PBS targets AK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AK1 fusion protein Ag6890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenylate kinase 1
别名 adenylate kinase 1, Adenylate kinase isoenzyme 1, Adenylate monophosphate kinase, AK 1, ATP:AMP phosphotransferase
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC001116
基因名称 AK1
Gene ID (NCBI) 203
RRIDAB_2225148
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00568
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AK1(Adenylate kinase isoenzyme 1), also known as ATP-AMP transphosphorylase 1. It is located in cytoplasm. This protein is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP 1. Exhibits nucleoside diphosphate kinase activity, catalyzing the production of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donor 2. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP. The molecular weight of AK1 is 22 kDa.