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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 15708-1-AP (same clone as 15708-1-PBS)

    WB analysis using 15708-1-AP (same clone as 15708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15708-1-AP (ACSL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 15708-1-AP (same clone as 15708-1-PBS)

    IP experiment of mouse brain using 15708-1-AP (same clone as 15708-1-PBS)

    IP result of anti-ACSL5 (IP:15708-1-AP, 4ug; Detection:15708-1-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15708-1-AP (ACSL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15708-1-AP (ACSL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

ACSL5 Polyclonal antibody, PBS Only

ACSL5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15708-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

LACS 5, FACL5, EC:6.2.1.3, EC:6.2.1.15, Arachidonate--CoA ligase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15708-1-PBS targets ACSL5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACSL5 fusion protein Ag8258 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase long-chain family member 5
别名 LACS 5, FACL5, EC:6.2.1.3, EC:6.2.1.15, Arachidonate--CoA ligase
计算分子量 76 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC007985
基因名称 ACSL5
Gene ID (NCBI) 51703
RRIDAB_2257881
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULC5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acyl-CoA synthetase 5, a member of the ACSL gene family that catalyzes the activation of long-chain fatty acids for lipid biosynthesis, is the only ACSL isoform that is both, located on mitochondria and functionally involved in enterocyte apoptosis.ACSL5 could play a role in promoting fatty acid-induced lipoapoptosis in hepatocytes as important mechanism in fatty liver-related disorders(PMID:20470896). ACSL5 is most abundant in liver, brown adipose tissue, and intestine and is located on both the mitochondrial membrane and endoplasmic reticulum(PMID:20798351). It has 3 isoforms produced by alternative splicing.