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验证数据展示

  • WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

    WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

    IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

    IHC staining of human liver using 55210-1-AP (same clone as 55210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

    WB analysis using 55210-1-AP (same clone as 55210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55210-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ACADM Polyclonal antibody, PBS Only

ACADM Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55210-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

ACAD, ACAD 1, ACAD1, EC:1.3.8.7, MCAD

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

55210-1-PBS targets ACADM in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
别名 ACAD, ACAD 1, ACAD1, EC:1.3.8.7, MCAD
计算分子量 47 kDa
观测分子量 45-50 kDa
GenBank蛋白编号NM_000016
基因名称 ACADM
Gene ID (NCBI) 34
RRIDAB_10837361
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11310
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

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