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CoraLite® Plus 488-conjugated ABCC5 Monoclonal antibody

ABCC5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H10F11

Cat No : CL488-67955

Print datasheet

Synonyms

ABC33, ABCC5, DKFZp686C1782, EST277145, MOAT C, MOATC, MRP5, pABC11, SMRP

验证数据展示

  • FC experiment of MCF-7 using CL488-67955

    FC experiment of MCF-7 using CL488-67955

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ABCC5 (CL488-67955, Clone:2H10F11) (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67955-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67955 targets ABCC5 in FC (Intra) applications and shows reactivity with Human, Rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Rat
Immunogen ABCC5 fusion protein Ag31674 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 5
Synonyms ABC33, ABCC5, DKFZp686C1782, EST277145, MOAT C, MOATC, MRP5, pABC11, SMRP
Calculated Molecular Weight 161 kDa
Observed Molecular Weight180-190kDa
GenBank Accession NumberNM_005688
Gene Symbol ABCC5
Gene ID (NCBI) 10057
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15440
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCC5, also named as MOAT-C, pABC11, SMRP and MRP5, belongs to the ABC transporter superfamily, ABCC family and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump which can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site is unclear.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ABCC5 antibody CL488-67955Download protocol
Standard Protocols
Click here to view our Standard Protocols