HRP-conjugated 6*His, His-Tag Monoclonal antibody

6*His, His-Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1


recombinant protein


WB and More (4)


HRP Fluorescent Dye



Cat No : HRP-66005

Print datasheet


6*His, 6*His, His Tag, 6*His, His-Tag, 6histag, 6XHis, HHHHHH, His tag, HISTIDINE TAGGED, HRP 6XHis, HRP HHHHHH, HRP His tag


Positive WB detected inRecombinant protein protein


Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery


HRP-66005 targets 6*His, His-Tag in WB, IP, ELISA, CoIP, Blocking assay applications and shows reactivity with recombinant protein samples.

Tested Applications WB
Cited Applications Blocking assay, CoIP, ELISA, IP, WB
Tested Reactivity recombinant protein
Immunogen 6xHis Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 6*His, His-Tag
Synonyms 6*His, 6*His, His Tag, 6*His, His-Tag, 6histag, 6XHis, HHHHHH, His tag, HISTIDINE TAGGED, HRP 6XHis, HRP HHHHHH, HRP His tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


Product Specific Protocols
WB protocol for HRP 6*His, His-Tag antibody HRP-66005Download protocol
Standard Protocols
Click here to view our Standard Protocols




Developing Covalent Protein Drugs via Proximity-Enabled Reactive Therapeutics.

Authors - Qingke Li


Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals.

Authors - Ling Ni


Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.

Authors - Yujia Wang

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun

Nat Chem Biol

Identification of a cellularly active SIRT6 allosteric activator.

Authors - Zhimin Huang

Nat Commun

Modular assembly of the principal microtubule nucleator γ-TuRC.

Authors - Martin Würtz