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验证数据展示

  • FC experiment of Transfected HEK-293T using 98672-2-RR

    FC experiment of Transfected HEK-293T using 98672-2-RR

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8a hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti-Whitlow/218 Linker Rabbit RecAb (98672-2-RR, Clone:252007G8), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9),and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of Transfected HEK-293T using 98672-2-RR

    FC experiment of Transfected HEK-293T using 98672-2-RR

    1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8a hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti-Whitlow/218 Linker Rabbit RecAb (98672-2-RR, Clone:252007G8) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue),and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • WB analysis using 98672-2-RR

    WB analysis using 98672-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 98672-2-RR (Whitlow/218 Linker antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Anti-Whitlow/218 Linker Rabbit Recombinant Antibody

Whitlow/218 Linker Uni-rAb® Recombinant Antibody for WB, FC
Cat No. 98672-2-RR

产品说明书

CloneNo. 252007G8

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

WB, FC

别名

218, Linker, Whitlow, 218 Linker, Whitlow Linker

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTransfected HEK-293T cells
Positive FC detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98672-2-RR targets Whitlow/218 Linker in WB, FC applications and shows reactivity with n/a samples.

经测试应用 WB, FC Application Description
经测试反应性 n/a
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Whitlow/218 Linker
别名 218, Linker, Whitlow, 218 Linker, Whitlow Linker
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

The 218 Linker (also known as the Whitlow linker) is a synthetic peptide sequence, connecting two protein domains, most commonly the Variable Heavy (VH) and Variable Light (VL) chains of an antibody, to form a stable single-chain variable fragment (scFv) (PMID: 8309948). Its standard amino acid sequence is GSTSGSGKPGSGEGSTKG.

实验方案

Product Specific Protocols
FC protocol for Whitlow/218 Linker antibody 98672-2-RRDownload protocol
WB protocol for Whitlow/218 Linker antibody 98672-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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