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验证数据展示

  • WB analysis using 86799-1-RR

    WB analysis using 86799-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86799-1-RR (UPK1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse bladder using 86799-1-RR

    IF Staining of mouse bladder using 86799-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse bladder tissue using UPK1A antibody (86799-1-RR, Clone: 251472C10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86799-1-RR

    Affinity and Kinetic Characterization of 86799-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86799-1-RR against Human UPK1A were performed. The affinity constant is below 1 pM.

UPK1A Recombinant monoclonal antibody

UPK1A Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 86799-1-RR

产品说明书

CloneNo. 251472C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

TSPAN21, Tetraspanin-21, Tspan-21, UP1a, UPIa

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse bladder tissue, rat bladder tissue
Positive IF-P detected inmouse bladder tissue
Planning an IHC experiment? We recommend our IHCeasy UPK1A Ready-To-Use IHC Kit. UPK1A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86799-1-RR targets UPK1A in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17356

Product name: Recombinant human UPK1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 111-230 aa of BC107098

Sequence: CITSYTHRDYMVSNPSLITKQMLTFYSADTDQGQELTRLWDRVMIEQECCGTSGPMDWVNFTSAFRAATPEVVFPWPPLCCRRTGNFIPLNEEGCRLGHMDYLFTKGCFEHIGHAIDSYT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 uroplakin 1A
别名 TSPAN21, Tetraspanin-21, Tspan-21, UP1a, UPIa
计算分子量 258 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC107098
基因名称 UPK1A
Gene ID (NCBI) 11045
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1a (UPK1A) belongs to the tetraspanin (TM4SF) family. UPK1A plays an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane.

实验方案

Product Specific Protocols
IF protocol for UPK1A antibody 86799-1-RRDownload protocol
WB protocol for UPK1A antibody 86799-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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