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验证数据展示

  • WB analysis using 86044-1-RR (same clone as 86044-1-PBS)

    WB analysis using 86044-1-RR (same clone as 86044-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86044-1-RR (PPP1R13L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86044-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02334-1

    Sandwich ELISA standard curve of MP02334-1

    Sandwich ELISA standard curve of MP02334-1, Human iASPP Recombinant Matched Antibody Pair - PBS only. 86044-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag13273. 86044-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP02334-1

    Sample test of MP02334-1

    The mean iASPP concentration was determined to be 32.51 ng/mL in PC-3 cell extract based on a 2.0 mg/mL extract load and 68.98 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load.

PPP1R13L Recombinant antibody, PBS Only (Detector)

PPP1R13L Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 86044-1-PBS

产品说明书

CloneNo. 250633E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Sandwich ELISA, Indirect ELISA, Sample test

别名

IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13B like protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PPP1R13L Ready-To-Use IHC Kit. PPP1R13L primary antibody included.

产品信息

86044-1-PBS targets PPP1R13L as part of a matched antibody pair:

MP02334-1: 86044-3-PBS capture and 86044-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 PPP1R13L fusion protein Ag13273 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 13 like
别名 IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13B like protein
计算分子量 89 kDa
观测分子量 110 kDa
GenBank蛋白编号BC064913
基因名称 PPP1R13L
Gene ID (NCBI) 10848
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WUF5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53-mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression.