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验证数据展示

  • WB analysis using 85390-2-RR

    WB analysis using 85390-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85390-2-RR (FCER1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85390-2-RR

    Affinity and Kinetic Characterization of 85390-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85390-2-RR against Human FCER1G were performed. The affinity constant is 1.16 nM.

FCER1G Recombinant antibody

FCER1G Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85390-2-RR

产品说明书

CloneNo. 242829E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

WB, ELISA

别名

242829E3, Fc epsilon RI gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, FceRI gamma

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells, RAW 264.7 cells, pig spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85390-2-RR targets FCER1G in WB, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, pig
免疫原 FCER1G fusion protein Ag4442 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
别名 242829E3, Fc epsilon RI gamma, Fc receptor gamma-chain, Fc-epsilon RI-gamma, FceRI gamma
计算分子量 87 aa, 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC033872
基因名称 FcRγ
Gene ID (NCBI) 2207
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30273
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FCER1G (Fc receptor gamma-chain, FcRgamma), also known as Fc-epsilon RI-gamma, or IgE Fc receptor subunit gamma (FceRI gamma), is a component of the high-affinity immunoglobulin E (IgE) receptor (Fc epsilon RI) which is a tetrameric complex of one alpha subunit, one beta subunit, and two disulfide-linked gamma subunits (PMID: 2146219; 2531187). Fc epsilon RI is present exclusively on mast cells and basophils, mediating allergic inflammatory signaling (PMID: 17438574). FCER1G is widely expressed and contains an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors (PMID: 19098920).

实验方案

Product Specific Protocols
WB protocol for FCER1G antibody 85390-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols